Incubation:Article Title: Memory Decline and Aberration of Synaptic Proteins in X-Linked Moesin Knockout Male Mice.
Article Snippet: .. The tissue lysates (30 μg) were electrophoresed and transferred to 0.45 μm nitrocellulose membranes, which were incubated with anti-GFAP (ab7260, 1:1,000, Abcam, Cambridge, MA, USA), anti-MAP2 (ab5392, 1:1,000, Abcam), anti-Sox2 (#4900, CST, 1:1,000), anti-DCX (#91954, CST, 1:1,000), anti-soluble NSF adapter protein 25 (SNAP25) (#5308, CST, 1:1,000), anti-postsynaptic density protein 95 (PSD95) (#3409, CST, 1:1,000), anti-phospho-PSD95 (#45737, CST, 1:1,000), or anti-moesin (#3146, CST, 1:1,000), anti-syntaxin1A (#18572, CST, 1:1,000), anti-synaptophysin (#5467, CST, 1:1,000), anti-CDK5 (#14145, CST, 1:1,000), anti-radixin (#2636, CST, 1:1,000), anti-synapsin 1 (#2312, CST, 1:1,000), anti-phospho-synapsin 1 (Ser9) (#2311, CST, 1:1,000), anti-phospho-synapsin 1 (Ser605) (#88246, CST, 1:1,000), anti-phospho-synapsin 1 (Ser549) (#PA1-4697, 1:1,000; Thermo Fisher Scientific, Waltham, MA, USA), antiERK (#4695, CST, 1:1,000), anti-phospho-ERK (#4370, CST, 1:1,000), anti-CREB (#9197, CST, 1:1,000), anti-phosphoCREB (#9190, CST, 1:1,000), anti-mammalian uncoordinated18 (MUNC18) (#13414, CST, 1:1,000), anti-phosphoMUNC18 (ab183484, 1:1,000, Abcam) anti-β-actin (#4967, CST, 1:1,000) for 16 hours at 4°C. .. After primary antibody incubation, each membrane was incubated with goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5,000; Thermo Fisher Scientific) or goat anti-mouse horseradish peroxidase-conjugated antibody (1:5,000; Thermo Fisher Scientific) for 1 hour at room temperature.
Article Title: Memory Decline and Aberration of Synaptic Proteins in X-Linked Moesin Knockout Male Mice
Article Snippet: .. The tissue lysates (30 μg) were electrophoresed and transferred to 0.45 μm nitrocellulose membranes, which were incubated with anti-GFAP (ab7260, 1:1,000, Abcam, Cambridge, MA, USA), anti-MAP2 (ab5392, 1:1,000, Abcam), anti-Sox2 (#4900, CST, 1:1,000), anti-DCX (#91954, CST, 1:1,000), anti-soluble NSF adapter protein 25 (SNAP25) (#5308, CST, 1:1,000), anti-postsynaptic density protein 95 (PSD95) (#3409, CST, 1:1,000), anti-phospho-PSD95 (#45737, CST, 1:1,000), or anti-moesin (#3146, CST, 1:1,000), anti-syntaxin1A (#18572, CST, 1:1,000), anti-synaptophysin (#5467, CST, 1:1,000), anti-CDK5 (#14145, CST, 1:1,000), anti-radixin (#2636, CST, 1:1,000), anti-synapsin 1 (#2312, CST, 1:1,000), anti-phospho-synapsin 1 (Ser9) (#2311, CST, 1:1,000), anti-phospho-synapsin 1 (Ser605) (#88246, CST, 1:1,000), anti-phospho-synapsin 1 (Ser549) (#PA1-4697, 1:1,000; Thermo Fisher Scientific, Waltham, MA, USA), anti-ERK (#4695, CST, 1:1,000), anti-phospho-ERK (#4370, CST, 1:1,000), anti-CREB (#9197, CST, 1:1,000), anti-phospho-CREB (#9190, CST, 1:1,000), anti-mammalian uncoordinated18 (MUNC18) (#13414, CST, 1:1,000), anti-phospho-MUNC18 (ab183484, 1:1,000, Abcam) anti-β-actin (#4967, CST, 1:1,000) for 16 hours at 4°C. .. After primary antibody incubation, each membrane was incubated with goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5,000; Thermo Fisher Scientific) or goat anti-mouse horseradish peroxidase-conjugated antibody (1:5,000; Thermo Fisher Scientific) for 1 hour at room temperature.
Tissue:Article Title: Memory Decline and Aberration of Synaptic Proteins in X-Linked Moesin Knockout Male Mice
Article Snippet: .. The tissue lysates (30 μg) were electrophoresed and transferred to 0.45 μm nitrocellulose membranes, which were incubated with anti-GFAP (ab7260, 1:1,000, Abcam, Cambridge, MA, USA), anti-MAP2 (ab5392, 1:1,000, Abcam), anti-Sox2 (#4900, CST, 1:1,000), anti-DCX (#91954, CST, 1:1,000), anti-soluble NSF adapter protein 25 (SNAP25) (#5308, CST, 1:1,000), anti-postsynaptic density protein 95 (PSD95) (#3409, CST, 1:1,000), anti-phospho-PSD95 (#45737, CST, 1:1,000), or anti-moesin (#3146, CST, 1:1,000), anti-syntaxin1A (#18572, CST, 1:1,000), anti-synaptophysin (#5467, CST, 1:1,000), anti-CDK5 (#14145, CST, 1:1,000), anti-radixin (#2636, CST, 1:1,000), anti-synapsin 1 (#2312, CST, 1:1,000), anti-phospho-synapsin 1 (Ser9) (#2311, CST, 1:1,000), anti-phospho-synapsin 1 (Ser605) (#88246, CST, 1:1,000), anti-phospho-synapsin 1 (Ser549) (#PA1-4697, 1:1,000; Thermo Fisher Scientific, Waltham, MA, USA), anti-ERK (#4695, CST, 1:1,000), anti-phospho-ERK (#4370, CST, 1:1,000), anti-CREB (#9197, CST, 1:1,000), anti-phospho-CREB (#9190, CST, 1:1,000), anti-mammalian uncoordinated18 (MUNC18) (#13414, CST, 1:1,000), anti-phospho-MUNC18 (ab183484, 1:1,000, Abcam) anti-β-actin (#4967, CST, 1:1,000) for 16 hours at 4°C. .. After primary antibody incubation, each membrane was incubated with goat anti-rabbit horseradish peroxidase-conjugated antibody (1:5,000; Thermo Fisher Scientific) or goat anti-mouse horseradish peroxidase-conjugated antibody (1:5,000; Thermo Fisher Scientific) for 1 hour at room temperature.
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